What do concentration assays estimate?

• A. pH of the solution.
• B. Concentration of the protein being used.
• C. Temperature of the solution.
• D. Molar absorptivity of the protein.

Answer: (B)

Concentration assays estimate how much protein is present in a solution. They produce a signal—often a color change—whose intensity is proportional to the amount of protein, and you use a standard curve to translate that signal into a concentration value. These assays focus on quantity, not on measuring pH or temperature. While spectroscopic approaches can relate absorbance to concentration using a constant like molar absorptivity, the assay itself is used to quantify protein amount, not to determine ε.